Error Analyzing Data File #2
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Further information is requested
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Hello BaltimoreBob, do you get any other output from the program before the error is output? It looks like there is an object (probably a table) somewhere in the code that isn't being made. If we can figure out which step it failed at I can see if I can fix it. |
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Hi Chris,
Here is the log on the analyze data error. It appears to be failing at the extracting MS/MS data step. I am including my .csv and the UserMods list as well and the method generated. I had to modify everything since my client wanted modifications on K and Nterminus. I can send you the .raw files if you would like but that will need an ftp or something. I did not use the priming run because my colleague said he couldn’t get that to work and this is my first time trying TOMAHAQ. Hope you had a nice holiday!
Appreciated,
Bob
Robert O’Meally, M.S., Proteomics Specialist
Mass Spectrometry and Proteomics Facility
Johns Hopkins School of Medicine
725 N. Wolfe Street, Room 510 Hunterian Bldg.
Baltimore, MD 21205
Ph: (410) 614-6968/Fax: (410) 614-7566
email: romeally@jhmi.edu<mailto:romeally@jhmi.edu>
From: CMRose3355 [mailto:notifications@github.com]
Sent: Sunday, July 08, 2018 12:02 AM
To: CMRose3355/TomahaqCompanionProgram <TomahaqCompanionProgram@noreply.github.com>
Cc: Bob O'Meally <romeally@jhmi.edu>; Author <author@noreply.github.com>
Subject: Re: [CMRose3355/TomahaqCompanionProgram] Error Analyzing Data File (#2)
Hello BaltimoreBob, do you get any other output from the program before the error is output? It looks like there is an object (probably a table) somewhere in the code that isn't being made. If we can figure out which step it failed at I can see if I can fix it.
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[12:51:54 PM] Version = 1.0.1.0
[12:53:09 PM] Importing Modifications
[12:53:09 PM] Determining Quantification Channels
[12:53:09 PM] Importing Target Peptides
[12:53:09 PM] Consolidating Data for GUI Tables
[12:53:09 PM] Building XML
[12:53:09 PM] Creating New Method
[12:53:11 PM] Writing method to D:\Data\2018\Client\RossC\RatovitskiT\180509\Notes\Peptides180628_DynamicN_Term_120Min.meth
[9:02:11 PM] Importing Modifications
[9:02:11 PM] Determining Quantification Channels
[9:02:11 PM] Importing Target Peptides
[9:02:11 PM] Opening Raw File
[9:02:12 PM] Mapping MS Scans
[9:02:12 PM] Extracting MS1 XICs
[9:02:15 PM] Extracting MS/MS Data
[9:02:15 PM] Error! Object reference not set to an instance of an object.
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Hello Bob, Can you please download the new version (1.0.2.0) and before you try the analysis to go the "Advanced Parameters" tab and check the "Print Debugging Messages" box. I have placed some print statements within the code that should help us narrow this down. Chris |
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Hi Chris,
I thought I was using the latest version. We downloaded from Github just two weeks ago. Which is the latest?
Bob
Robert O’Meally, M.S., Proteomics Specialist
Mass Spectrometry and Proteomics Facility
Johns Hopkins School of Medicine
725 N. Wolfe Street, Room 510 Hunterian Bldg.
Baltimore, MD 21205
Ph: (410) 614-6968/Fax: (410) 614-7566
email: romeally@jhmi.edu<mailto:romeally@jhmi.edu>
From: CMRose3355 [mailto:notifications@github.com]
Sent: Sunday, July 08, 2018 11:47 PM
To: CMRose3355/TomahaqCompanionProgram <TomahaqCompanionProgram@noreply.github.com>
Cc: Bob O'Meally <romeally@jhmi.edu>; Author <author@noreply.github.com>
Subject: Re: [CMRose3355/TomahaqCompanionProgram] Error Analyzing Data File (#2)
Hello Bob,
Can you please download the new version and before you try the analysis to go the "Advanced Parameters" tab and check the "Print Debugging Messages" box. I have placed some print statements within the code that should help us narrow this down.
Chris
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Oh OK I see the new one now. Downloading. I have a standard that I labeled with TMT 0 and then a second set at 1:2:5:10 with TMT 10. I use this to verify the method is working. I will run this with the new version later today. Thanks!
Bob
Ps. So is there any way to do the analysis on an offline computer? We are a core lab and when the runs are done we still can’t share the instrument since we have to analyze data on the acquisition computer. This would be a really nice feature if it does not exist already. I will let you know when I have rerun with the debugging.
Robert O’Meally, M.S., Proteomics Specialist
Mass Spectrometry and Proteomics Facility
Johns Hopkins School of Medicine
725 N. Wolfe Street, Room 510 Hunterian Bldg.
Baltimore, MD 21205
Ph: (410) 614-6968/Fax: (410) 614-7566
email: romeally@jhmi.edu<mailto:romeally@jhmi.edu>
From: CMRose3355 [mailto:notifications@github.com]
Sent: Sunday, July 08, 2018 11:47 PM
To: CMRose3355/TomahaqCompanionProgram <TomahaqCompanionProgram@noreply.github.com>
Cc: Bob O'Meally <romeally@jhmi.edu>; Author <author@noreply.github.com>
Subject: Re: [CMRose3355/TomahaqCompanionProgram] Error Analyzing Data File (#2)
Hello Bob,
Can you please download the new version and before you try the analysis to go the "Advanced Parameters" tab and check the "Print Debugging Messages" box. I have placed some print statements within the code that should help us narrow this down.
Chris
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You are receiving this because you authored the thread.
Reply to this email directly, view it on GitHub<#2 (comment)>, or mute the thread<https://github.com/notifications/unsubscribe-auth/Am0FZZeqawoXHYR-TyY0VBPZgcdcBf9tks5uEtI3gaJpZM4U9VB6>.
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Yes, you can do the analysis on an offline computer. The automated method creation is the only feature that needs an instrument computer. All other functions should work on a offline computer. |
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Oh…I was told by my colleague you had to analyze on the instrument computer. Maybe he is just an instrument hog! So with the latest version it fails to create my method. Here is the error log when I execute. These are otherwise the same parameters that created my old method.
Thanks!
Bob
…--------------------------------------------------------
Robert O’Meally, M.S., Proteomics Specialist
Mass Spectrometry and Proteomics Facility
Johns Hopkins School of Medicine
725 N. Wolfe Street, Room 510 Hunterian Bldg.
Baltimore, MD 21205
Ph: (410) 614-6968/Fax: (410) 614-7566
email: romeally@jhmi.edu<mailto:romeally@jhmi.edu>
From: CMRose3355 [mailto:notifications@github.com]
Sent: Monday, July 09, 2018 8:00 AM
To: CMRose3355/TomahaqCompanionProgram
Cc: Bob O'Meally; Author
Subject: Re: [CMRose3355/TomahaqCompanionProgram] Error Analyzing Data File (#2)
Yes, you can do the analysis on an offline computer. The automated method creation is the only feature that needs an instrument computer. All other functions should work on a offline computer.
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[9:54:26 AM] Version = 1.0.2.0
[9:57:05 AM] Importing Modifications
[9:57:05 AM] Determining Quantification Channels
[9:57:05 AM] Importing Target Peptides
[9:57:05 AM] Consolidating Data for GUI Tables
[9:57:05 AM] Building XML
[9:57:05 AM] Creating New Method
[9:57:06 AM] Error! Could not load file or assembly 'file:///C:\Xcalibur\TOMAHAQ_180709\TomahaqCompanionProgram-master\TomahaqCompanionProgram-master\TomahaqCompanion\Thermo.TNG.MethodXMLFactory.dll' or one of its dependencies. Operation is not supported. (Exception from HRESULT: 0x80131515)
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I believe that this is related to the unzipping issue that is discussed on the other "issue" raised here. Try that fix and see if the method can be made. |
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Hi Chris,
That must have been it. The cloned program made the method. So where should I see the debugging errors? I have not run the newest method but it was created successfully.
Bob
Robert O’Meally, M.S., Proteomics Specialist
Mass Spectrometry and Proteomics Facility
Johns Hopkins School of Medicine
725 N. Wolfe Street, Room 510 Hunterian Bldg.
Baltimore, MD 21205
Ph: (410) 614-6968/Fax: (410) 614-7566
email: romeally@jhmi.edu<mailto:romeally@jhmi.edu>
From: CMRose3355 [mailto:notifications@github.com]
Sent: Monday, July 09, 2018 12:13 PM
To: CMRose3355/TomahaqCompanionProgram <TomahaqCompanionProgram@noreply.github.com>
Cc: Bob O'Meally <romeally@jhmi.edu>; Author <author@noreply.github.com>
Subject: Re: [CMRose3355/TomahaqCompanionProgram] Error Analyzing Data File (#2)
I believe that this is related to the unzipping issue that is discussed on the other "issue" raised here<#1>.
Try that fix and see if the method can be made.
—
You are receiving this because you authored the thread.
Reply to this email directly, view it on GitHub<#2 (comment)>, or mute the thread<https://github.com/notifications/unsubscribe-auth/Am0FZTeag0X6JVKPzyCgHxKoxReTAVrdks5uE4EAgaJpZM4U9VB6>.
[1:08:33 PM] Version = 1.0.2.0
[1:09:08 PM] Importing Modifications
[1:09:08 PM] Determining Quantification Channels
[1:09:08 PM] Importing Target Peptides
[1:09:08 PM] Consolidating Data for GUI Tables
[1:09:08 PM] Building XML
[1:09:08 PM] Creating New Method
[1:09:10 PM] Writing method to D:\Data\2018\Client\RossC\RatovitskiT\180509\Notes\Peptides180709_DynamicN_Term_120Min.meth
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I believe that you mentioned the error was occurring during analysis of data. I would try to analyze the same data that was producing the error previously. I currently only placed messages within the code that does data analysis, and not the code that does method creation. |
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Gotcha…here it is…seems like on or after scan 1555 is crashing it? Where should I look to correct?
Robert O’Meally, M.S., Proteomics Specialist
Mass Spectrometry and Proteomics Facility
Johns Hopkins School of Medicine
725 N. Wolfe Street, Room 510 Hunterian Bldg.
Baltimore, MD 21205
Ph: (410) 614-6968/Fax: (410) 614-7566
email: romeally@jhmi.edu<mailto:romeally@jhmi.edu>
From: CMRose3355 [mailto:notifications@github.com]
Sent: Monday, July 09, 2018 1:16 PM
To: CMRose3355/TomahaqCompanionProgram <TomahaqCompanionProgram@noreply.github.com>
Cc: Bob O'Meally <romeally@jhmi.edu>; Author <author@noreply.github.com>
Subject: Re: [CMRose3355/TomahaqCompanionProgram] Error Analyzing Data File (#2)
I believe that you mentioned the error was occurring during analysis of data. I would try to analyze the same data that was producing the error previously. I currently only placed messages within the code that does data analysis, and not the code that does method creation.
—
You are receiving this because you authored the thread.
Reply to this email directly, view it on GitHub<#2 (comment)>, or mute the thread<https://github.com/notifications/unsubscribe-auth/Am0FZbXx-qt2vVtCtYQDtslhYiGxTWo0ks5uE4_EgaJpZM4U9VB6>.
[1:08:33 PM] Version = 1.0.2.0
[1:09:08 PM] Importing Modifications
[1:09:08 PM] Determining Quantification Channels
[1:09:08 PM] Importing Target Peptides
[1:09:08 PM] Consolidating Data for GUI Tables
[1:09:08 PM] Building XML
[1:09:08 PM] Creating New Method
[1:09:10 PM] Writing method to D:\Data\2018\Client\RossC\RatovitskiT\180509\Notes\Peptides180709_DynamicN_Term_120Min.meth
[1:14:58 PM] Importing Modifications
[1:14:58 PM] Determining Quantification Channels
[1:14:58 PM] Importing Target Peptides
[1:14:58 PM] Opening Raw File
[1:14:58 PM] Mapping MS Scans
[1:14:59 PM] Extracting MS1 XICs
[1:15:02 PM] Extracting MS/MS Data
[1:15:02 PM] Extracting MS1 Data
[1:15:02 PM] Analyzing Scan Number 1015
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1016
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1018
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1020
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1024
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1144
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1148
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1197
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1199
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1200
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1203
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1204
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1206
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1208
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1210
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1211
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1213
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1215
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1217
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1219
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1221
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1223
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1225
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1227
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1229
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1231
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1233
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Extracting MS2/3 Data
[1:15:02 PM] Adding Target Data
[1:15:02 PM] Extracted MS2/3 Data
[1:15:02 PM] Analyzing Scan Number 1236
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1238
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1240
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1242
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1244
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1246
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1248
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1250
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1251
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1253
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1254
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1256
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1258
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1260
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1261
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1262
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1264
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Extracting MS2/3 Data
[1:15:02 PM] Adding Target Data
[1:15:02 PM] Extracted MS2/3 Data
[1:15:02 PM] Analyzing Scan Number 1265
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1268
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Extracting MS2/3 Data
[1:15:02 PM] Adding Target Data
[1:15:02 PM] Extracted MS2/3 Data
[1:15:02 PM] Analyzing Scan Number 1269
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1271
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1273
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Extracting MS2/3 Data
[1:15:02 PM] Adding Target Data
[1:15:02 PM] Extracted MS2/3 Data
[1:15:02 PM] Analyzing Scan Number 1274
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1277
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Extracting MS2/3 Data
[1:15:02 PM] Adding Target Data
[1:15:02 PM] Extracted MS2/3 Data
[1:15:02 PM] Analyzing Scan Number 1278
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1281
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Extracting MS2/3 Data
[1:15:02 PM] Adding Target Data
[1:15:02 PM] Extracted MS2/3 Data
[1:15:02 PM] Analyzing Scan Number 1282
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1285
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Extracting MS2/3 Data
[1:15:02 PM] Adding Target Data
[1:15:02 PM] Extracted MS2/3 Data
[1:15:02 PM] Analyzing Scan Number 1286
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1289
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Extracting MS2/3 Data
[1:15:02 PM] Adding Target Data
[1:15:02 PM] Extracted MS2/3 Data
[1:15:02 PM] Analyzing Scan Number 1290
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Analyzing Scan Number 1293
[1:15:02 PM] Target Peptide Found
[1:15:02 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:02 PM] Querying Target Data
[1:15:02 PM] Extracting MS2/3 Data
[1:15:02 PM] Adding Target Data
[1:15:02 PM] Extracted MS2/3 Data
[1:15:03 PM] Analyzing Scan Number 1294
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Analyzing Scan Number 1296
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Analyzing Scan Number 1298
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Extracting MS2/3 Data
[1:15:03 PM] Adding Target Data
[1:15:03 PM] Extracted MS2/3 Data
[1:15:03 PM] Analyzing Scan Number 1301
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Analyzing Scan Number 1302
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Analyzing Scan Number 1304
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Analyzing Scan Number 1306
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Analyzing Scan Number 1308
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Analyzing Scan Number 1311
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Analyzing Scan Number 1313
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Analyzing Scan Number 1315
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Analyzing Scan Number 1317
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Analyzing Scan Number 1323
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Analyzing Scan Number 1335
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Analyzing Scan Number 1342
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Analyzing Scan Number 1345
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Analyzing Scan Number 1348
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Analyzing Scan Number 1396
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Analyzing Scan Number 1398
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Analyzing Scan Number 1400
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Analyzing Scan Number 1405
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Analyzing Scan Number 1407
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Analyzing Scan Number 1409
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Analyzing Scan Number 1411
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Analyzing Scan Number 1415
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Analyzing Scan Number 1417
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Analyzing Scan Number 1419
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Analyzing Scan Number 1421
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Analyzing Scan Number 1423
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Analyzing Scan Number 1428
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Analyzing Scan Number 1430
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Analyzing Scan Number 1438
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Analyzing Scan Number 1472
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Analyzing Scan Number 1477
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Analyzing Scan Number 1480
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Extracting MS2/3 Data
[1:15:03 PM] Adding Target Data
[1:15:03 PM] Extracted MS2/3 Data
[1:15:03 PM] Analyzing Scan Number 1483
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Extracting MS2/3 Data
[1:15:03 PM] Adding Target Data
[1:15:03 PM] Extracted MS2/3 Data
[1:15:03 PM] Analyzing Scan Number 1486
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Extracting MS2/3 Data
[1:15:03 PM] Adding Target Data
[1:15:03 PM] Extracted MS2/3 Data
[1:15:03 PM] Analyzing Scan Number 1489
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Extracting MS2/3 Data
[1:15:03 PM] Adding Target Data
[1:15:03 PM] Extracted MS2/3 Data
[1:15:03 PM] Analyzing Scan Number 1490
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Analyzing Scan Number 1493
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Extracting MS2/3 Data
[1:15:03 PM] Adding Target Data
[1:15:03 PM] Extracted MS2/3 Data
[1:15:03 PM] Analyzing Scan Number 1496
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Extracting MS2/3 Data
[1:15:03 PM] Adding Target Data
[1:15:03 PM] Extracted MS2/3 Data
[1:15:03 PM] Analyzing Scan Number 1497
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Analyzing Scan Number 1500
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Extracting MS2/3 Data
[1:15:03 PM] Adding Target Data
[1:15:03 PM] Extracted MS2/3 Data
[1:15:03 PM] Analyzing Scan Number 1503
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Extracting MS2/3 Data
[1:15:03 PM] Adding Target Data
[1:15:03 PM] Extracted MS2/3 Data
[1:15:03 PM] Analyzing Scan Number 1506
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Extracting MS2/3 Data
[1:15:03 PM] Adding Target Data
[1:15:03 PM] Extracted MS2/3 Data
[1:15:03 PM] Analyzing Scan Number 1509
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Extracting MS2/3 Data
[1:15:03 PM] Adding Target Data
[1:15:03 PM] Extracted MS2/3 Data
[1:15:03 PM] Analyzing Scan Number 1512
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Extracting MS2/3 Data
[1:15:03 PM] Adding Target Data
[1:15:03 PM] Extracted MS2/3 Data
[1:15:03 PM] Analyzing Scan Number 1513
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Analyzing Scan Number 1516
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Extracting MS2/3 Data
[1:15:03 PM] Adding Target Data
[1:15:03 PM] Extracted MS2/3 Data
[1:15:03 PM] Analyzing Scan Number 1519
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Extracting MS2/3 Data
[1:15:03 PM] Adding Target Data
[1:15:03 PM] Extracted MS2/3 Data
[1:15:03 PM] Analyzing Scan Number 1522
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Extracting MS2/3 Data
[1:15:03 PM] Adding Target Data
[1:15:03 PM] Extracted MS2/3 Data
[1:15:03 PM] Analyzing Scan Number 1525
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Extracting MS2/3 Data
[1:15:03 PM] Adding Target Data
[1:15:03 PM] Extracted MS2/3 Data
[1:15:03 PM] Analyzing Scan Number 1528
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Extracting MS2/3 Data
[1:15:03 PM] Adding Target Data
[1:15:03 PM] Extracted MS2/3 Data
[1:15:03 PM] Analyzing Scan Number 1529
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Analyzing Scan Number 1532
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Extracting MS2/3 Data
[1:15:03 PM] Adding Target Data
[1:15:03 PM] Extracted MS2/3 Data
[1:15:03 PM] Analyzing Scan Number 1535
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Extracting MS2/3 Data
[1:15:03 PM] Adding Target Data
[1:15:03 PM] Extracted MS2/3 Data
[1:15:03 PM] Analyzing Scan Number 1538
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Extracting MS2/3 Data
[1:15:03 PM] Adding Target Data
[1:15:03 PM] Extracted MS2/3 Data
[1:15:03 PM] Analyzing Scan Number 1541
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Extracting MS2/3 Data
[1:15:03 PM] Adding Target Data
[1:15:03 PM] Extracted MS2/3 Data
[1:15:03 PM] Analyzing Scan Number 1542
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Analyzing Scan Number 1545
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Extracting MS2/3 Data
[1:15:03 PM] Adding Target Data
[1:15:03 PM] Extracted MS2/3 Data
[1:15:03 PM] Analyzing Scan Number 1548
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Extracting MS2/3 Data
[1:15:03 PM] Adding Target Data
[1:15:03 PM] Extracted MS2/3 Data
[1:15:03 PM] Analyzing Scan Number 1549
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Analyzing Scan Number 1552
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Extracting MS2/3 Data
[1:15:03 PM] Adding Target Data
[1:15:03 PM] Extracted MS2/3 Data
[1:15:03 PM] Analyzing Scan Number 1555
[1:15:03 PM] Target Peptide Found
[1:15:03 PM] Spectrum Retrieved, Adding Trigger Data
[1:15:03 PM] Querying Target Data
[1:15:03 PM] Extracting MS2/3 Data
[1:15:03 PM] Adding Target Data
[1:15:03 PM] Error! Object reference not set to an instance of an object.
|
|
Ok I added in more messages to narrow it down a bit more. It appears that something is missing from the raw file. You can download the version I just published and try the analysis again. One thing to look for is to make sure that scan 1555 is an MS2 than has an MS3 associated with it. You can also check the scan header to see if the fields for SPS ions are empty or not. What version of Xcalibur and Tune are you running? |
|
That version didn’t give any new information. Here is a slicer of the .RAW file. Scan 1550 is scan 1 on here so 1555 is scan 6. This appears to be where or shortly before the error is occurring.
Thanks,
Bob
Robert O’Meally, M.S., Proteomics Specialist
Mass Spectrometry and Proteomics Facility
Johns Hopkins School of Medicine
725 N. Wolfe Street, Room 510 Hunterian Bldg.
Baltimore, MD 21205
Ph: (410) 614-6968/Fax: (410) 614-7566
email: romeally@jhmi.edu<mailto:romeally@jhmi.edu>
From: CMRose3355 [mailto:notifications@github.com]
Sent: Monday, July 09, 2018 1:49 PM
To: CMRose3355/TomahaqCompanionProgram <TomahaqCompanionProgram@noreply.github.com>
Cc: Bob O'Meally <romeally@jhmi.edu>; Author <author@noreply.github.com>
Subject: Re: [CMRose3355/TomahaqCompanionProgram] Error Analyzing Data File (#2)
Ok I added in more messages to narrow it down a bit more. It appears that something is missing from the raw file. You can download the version I just published and try the analysis again.
One thing to look for is to make sure that scan 1555 is an MS2 than has an MS3 associated with it. You can also check the scan header to see if the fields for SPS ions are empty or not.
What version of Xcalibur and Tune are you running?
—
You are receiving this because you authored the thread.
Reply to this email directly, view it on GitHub<#2 (comment)>, or mute the thread<https://github.com/notifications/unsubscribe-auth/Am0FZV6ncZUUA0KyjCvYgEyQTNFQflBFks5uE5eggaJpZM4U9VB6>.
[2:08:10 PM] Importing Modifications
[2:08:10 PM] Determining Quantification Channels
[2:08:10 PM] Importing Target Peptides
[2:08:10 PM] Opening Raw File
[2:08:10 PM] Mapping MS Scans
[2:08:10 PM] Extracting MS1 XICs
[2:08:13 PM] Extracting MS/MS Data
[2:08:13 PM] Extracting MS1 Data
[2:08:13 PM] Analyzing Scan Number 1015
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:13 PM] Analyzing Scan Number 1016
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:13 PM] Analyzing Scan Number 1018
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:13 PM] Analyzing Scan Number 1020
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:13 PM] Analyzing Scan Number 1024
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:13 PM] Analyzing Scan Number 1144
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:13 PM] Analyzing Scan Number 1148
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:13 PM] Analyzing Scan Number 1197
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:13 PM] Analyzing Scan Number 1199
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:13 PM] Analyzing Scan Number 1200
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:13 PM] Analyzing Scan Number 1203
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:13 PM] Analyzing Scan Number 1204
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:13 PM] Analyzing Scan Number 1206
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:13 PM] Analyzing Scan Number 1208
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:13 PM] Analyzing Scan Number 1210
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:13 PM] Analyzing Scan Number 1211
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:13 PM] Analyzing Scan Number 1213
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:13 PM] Analyzing Scan Number 1215
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:13 PM] Analyzing Scan Number 1217
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:13 PM] Analyzing Scan Number 1219
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:13 PM] Analyzing Scan Number 1221
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:13 PM] Analyzing Scan Number 1223
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:13 PM] Analyzing Scan Number 1225
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:13 PM] Analyzing Scan Number 1227
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:13 PM] Analyzing Scan Number 1229
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:13 PM] Analyzing Scan Number 1231
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:13 PM] Analyzing Scan Number 1233
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:13 PM] Extracting MS2/3 Data
[2:08:13 PM] Adding Target Data 2
[2:08:13 PM] Added Target Data 2
[2:08:13 PM] Extracted MS2/3 Data
[2:08:13 PM] Analyzing Scan Number 1236
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:13 PM] Analyzing Scan Number 1238
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:13 PM] Analyzing Scan Number 1240
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:13 PM] Analyzing Scan Number 1242
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:13 PM] Analyzing Scan Number 1244
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:13 PM] Analyzing Scan Number 1246
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:13 PM] Analyzing Scan Number 1248
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:13 PM] Analyzing Scan Number 1250
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:13 PM] Analyzing Scan Number 1251
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:13 PM] Analyzing Scan Number 1253
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:13 PM] Analyzing Scan Number 1254
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:13 PM] Analyzing Scan Number 1256
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:13 PM] Analyzing Scan Number 1258
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:13 PM] Analyzing Scan Number 1260
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:13 PM] Analyzing Scan Number 1261
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:13 PM] Analyzing Scan Number 1262
[2:08:13 PM] Target Peptide Found
[2:08:13 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:13 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1264
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Extracting MS2/3 Data
[2:08:14 PM] Adding Target Data 2
[2:08:14 PM] Added Target Data 2
[2:08:14 PM] Extracted MS2/3 Data
[2:08:14 PM] Analyzing Scan Number 1265
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1268
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Extracting MS2/3 Data
[2:08:14 PM] Adding Target Data 2
[2:08:14 PM] Added Target Data 2
[2:08:14 PM] Extracted MS2/3 Data
[2:08:14 PM] Analyzing Scan Number 1269
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1271
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1273
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Extracting MS2/3 Data
[2:08:14 PM] Adding Target Data 2
[2:08:14 PM] Added Target Data 2
[2:08:14 PM] Extracted MS2/3 Data
[2:08:14 PM] Analyzing Scan Number 1274
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1277
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Extracting MS2/3 Data
[2:08:14 PM] Adding Target Data 2
[2:08:14 PM] Added Target Data 2
[2:08:14 PM] Extracted MS2/3 Data
[2:08:14 PM] Analyzing Scan Number 1278
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1281
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Extracting MS2/3 Data
[2:08:14 PM] Adding Target Data 2
[2:08:14 PM] Added Target Data 2
[2:08:14 PM] Extracted MS2/3 Data
[2:08:14 PM] Analyzing Scan Number 1282
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1285
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Extracting MS2/3 Data
[2:08:14 PM] Adding Target Data 2
[2:08:14 PM] Added Target Data 2
[2:08:14 PM] Extracted MS2/3 Data
[2:08:14 PM] Analyzing Scan Number 1286
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1289
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Extracting MS2/3 Data
[2:08:14 PM] Adding Target Data 2
[2:08:14 PM] Added Target Data 2
[2:08:14 PM] Extracted MS2/3 Data
[2:08:14 PM] Analyzing Scan Number 1290
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1293
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Extracting MS2/3 Data
[2:08:14 PM] Adding Target Data 2
[2:08:14 PM] Added Target Data 2
[2:08:14 PM] Extracted MS2/3 Data
[2:08:14 PM] Analyzing Scan Number 1294
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1296
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1298
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Extracting MS2/3 Data
[2:08:14 PM] Adding Target Data 2
[2:08:14 PM] Added Target Data 2
[2:08:14 PM] Extracted MS2/3 Data
[2:08:14 PM] Analyzing Scan Number 1301
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1302
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1304
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1306
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1308
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1311
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1313
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1315
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1317
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1323
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1335
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1342
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1345
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1348
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1396
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1398
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1400
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1405
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1407
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1409
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1411
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1415
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1417
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1419
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1421
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1423
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1428
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1430
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1438
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1472
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1477
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1480
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Extracting MS2/3 Data
[2:08:14 PM] Adding Target Data 2
[2:08:14 PM] Added Target Data 2
[2:08:14 PM] Extracted MS2/3 Data
[2:08:14 PM] Analyzing Scan Number 1483
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Extracting MS2/3 Data
[2:08:14 PM] Adding Target Data 2
[2:08:14 PM] Added Target Data 2
[2:08:14 PM] Extracted MS2/3 Data
[2:08:14 PM] Analyzing Scan Number 1486
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Extracting MS2/3 Data
[2:08:14 PM] Adding Target Data 2
[2:08:14 PM] Added Target Data 2
[2:08:14 PM] Extracted MS2/3 Data
[2:08:14 PM] Analyzing Scan Number 1489
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Extracting MS2/3 Data
[2:08:14 PM] Adding Target Data 2
[2:08:14 PM] Added Target Data 2
[2:08:14 PM] Extracted MS2/3 Data
[2:08:14 PM] Analyzing Scan Number 1490
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1493
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Extracting MS2/3 Data
[2:08:14 PM] Adding Target Data 2
[2:08:14 PM] Added Target Data 2
[2:08:14 PM] Extracted MS2/3 Data
[2:08:14 PM] Analyzing Scan Number 1496
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Extracting MS2/3 Data
[2:08:14 PM] Adding Target Data 2
[2:08:14 PM] Added Target Data 2
[2:08:14 PM] Extracted MS2/3 Data
[2:08:14 PM] Analyzing Scan Number 1497
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1500
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Extracting MS2/3 Data
[2:08:14 PM] Adding Target Data 2
[2:08:14 PM] Added Target Data 2
[2:08:14 PM] Extracted MS2/3 Data
[2:08:14 PM] Analyzing Scan Number 1503
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Extracting MS2/3 Data
[2:08:14 PM] Adding Target Data 2
[2:08:14 PM] Added Target Data 2
[2:08:14 PM] Extracted MS2/3 Data
[2:08:14 PM] Analyzing Scan Number 1506
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Extracting MS2/3 Data
[2:08:14 PM] Adding Target Data 2
[2:08:14 PM] Added Target Data 2
[2:08:14 PM] Extracted MS2/3 Data
[2:08:14 PM] Analyzing Scan Number 1509
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Extracting MS2/3 Data
[2:08:14 PM] Adding Target Data 2
[2:08:14 PM] Added Target Data 2
[2:08:14 PM] Extracted MS2/3 Data
[2:08:14 PM] Analyzing Scan Number 1512
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Extracting MS2/3 Data
[2:08:14 PM] Adding Target Data 2
[2:08:14 PM] Added Target Data 2
[2:08:14 PM] Extracted MS2/3 Data
[2:08:14 PM] Analyzing Scan Number 1513
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1516
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Extracting MS2/3 Data
[2:08:14 PM] Adding Target Data 2
[2:08:14 PM] Added Target Data 2
[2:08:14 PM] Extracted MS2/3 Data
[2:08:14 PM] Analyzing Scan Number 1519
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Extracting MS2/3 Data
[2:08:14 PM] Adding Target Data 2
[2:08:14 PM] Added Target Data 2
[2:08:14 PM] Extracted MS2/3 Data
[2:08:14 PM] Analyzing Scan Number 1522
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Extracting MS2/3 Data
[2:08:14 PM] Adding Target Data 2
[2:08:14 PM] Added Target Data 2
[2:08:14 PM] Extracted MS2/3 Data
[2:08:14 PM] Analyzing Scan Number 1525
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Extracting MS2/3 Data
[2:08:14 PM] Adding Target Data 2
[2:08:14 PM] Added Target Data 2
[2:08:14 PM] Extracted MS2/3 Data
[2:08:14 PM] Analyzing Scan Number 1528
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Extracting MS2/3 Data
[2:08:14 PM] Adding Target Data 2
[2:08:14 PM] Added Target Data 2
[2:08:14 PM] Extracted MS2/3 Data
[2:08:14 PM] Analyzing Scan Number 1529
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1532
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Extracting MS2/3 Data
[2:08:14 PM] Adding Target Data 2
[2:08:14 PM] Added Target Data 2
[2:08:14 PM] Extracted MS2/3 Data
[2:08:14 PM] Analyzing Scan Number 1535
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Extracting MS2/3 Data
[2:08:14 PM] Adding Target Data 2
[2:08:14 PM] Added Target Data 2
[2:08:14 PM] Extracted MS2/3 Data
[2:08:14 PM] Analyzing Scan Number 1538
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Extracting MS2/3 Data
[2:08:14 PM] Adding Target Data 2
[2:08:14 PM] Added Target Data 2
[2:08:14 PM] Extracted MS2/3 Data
[2:08:14 PM] Analyzing Scan Number 1541
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Extracting MS2/3 Data
[2:08:14 PM] Adding Target Data 2
[2:08:14 PM] Added Target Data 2
[2:08:14 PM] Extracted MS2/3 Data
[2:08:14 PM] Analyzing Scan Number 1542
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1545
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Extracting MS2/3 Data
[2:08:14 PM] Adding Target Data 2
[2:08:14 PM] Added Target Data 2
[2:08:14 PM] Extracted MS2/3 Data
[2:08:14 PM] Analyzing Scan Number 1548
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Extracting MS2/3 Data
[2:08:14 PM] Adding Target Data 2
[2:08:14 PM] Added Target Data 2
[2:08:14 PM] Extracted MS2/3 Data
[2:08:14 PM] Analyzing Scan Number 1549
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Analyzing Scan Number 1552
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Extracting MS2/3 Data
[2:08:14 PM] Adding Target Data 2
[2:08:14 PM] Added Target Data 2
[2:08:14 PM] Extracted MS2/3 Data
[2:08:14 PM] Analyzing Scan Number 1555
[2:08:14 PM] Target Peptide Found
[2:08:14 PM] Spectrum Retrieved, Adding Trigger Data
[2:08:14 PM] Querying Target Data
[2:08:14 PM] Extracting MS2/3 Data
[2:08:14 PM] Adding Target Data 1
[2:08:14 PM] Error! Object reference not set to an instance of an object.
|
|
Hello Bob, Please try the newest version again. The last trial helped be narrow in on the issue. There is a small chunk of code that could be the issue and hopefully the newest version will demonstrate where the error is. |
|
Here you go. Looks like it gets to 1558 now.
Robert O’Meally, M.S., Proteomics Specialist
Mass Spectrometry and Proteomics Facility
Johns Hopkins School of Medicine
725 N. Wolfe Street, Room 510 Hunterian Bldg.
Baltimore, MD 21205
Ph: (410) 614-6968/Fax: (410) 614-7566
email: romeally@jhmi.edu<mailto:romeally@jhmi.edu>
From: CMRose3355 [mailto:notifications@github.com]
Sent: Monday, July 09, 2018 3:19 PM
To: CMRose3355/TomahaqCompanionProgram <TomahaqCompanionProgram@noreply.github.com>
Cc: Bob O'Meally <romeally@jhmi.edu>; Author <author@noreply.github.com>
Subject: Re: [CMRose3355/TomahaqCompanionProgram] Error Analyzing Data File (#2)
Hello Bob,
Please try the newest version again. The last trial helped be narrow in on the issue. There is a small chunk of code that could be the issue and hopefully the newest version will demonstrate where the error is.
—
You are receiving this because you authored the thread.
Reply to this email directly, view it on GitHub<#2 (comment)>, or mute the thread<https://github.com/notifications/unsubscribe-auth/Am0FZfmZDbEhqYKeZkTyg6FVqUowGePuks5uE6yzgaJpZM4U9VB6>.
[3:42:09 PM] Version = 1.0.2.0
[3:42:50 PM] Importing Modifications
[3:42:50 PM] Determining Quantification Channels
[3:42:50 PM] Importing Target Peptides
[3:42:50 PM] Opening Raw File
[3:42:51 PM] Mapping MS Scans
[3:42:51 PM] Extracting MS1 XICs
[3:42:54 PM] Extracting MS/MS Data
[3:42:54 PM] Analyzing Scan Number 1015
[3:42:54 PM] Analyzing Scan Number 1016
[3:42:54 PM] Analyzing Scan Number 1018
[3:42:54 PM] Analyzing Scan Number 1020
[3:42:54 PM] Analyzing Scan Number 1024
[3:42:54 PM] Analyzing Scan Number 1144
[3:42:54 PM] Analyzing Scan Number 1148
[3:42:54 PM] Analyzing Scan Number 1197
[3:42:54 PM] Analyzing Scan Number 1199
[3:42:54 PM] Analyzing Scan Number 1200
[3:42:54 PM] Analyzing Scan Number 1203
[3:42:54 PM] Analyzing Scan Number 1204
[3:42:54 PM] Analyzing Scan Number 1206
[3:42:54 PM] Analyzing Scan Number 1208
[3:42:54 PM] Analyzing Scan Number 1210
[3:42:54 PM] Analyzing Scan Number 1211
[3:42:54 PM] Analyzing Scan Number 1213
[3:42:54 PM] Analyzing Scan Number 1215
[3:42:54 PM] Analyzing Scan Number 1217
[3:42:54 PM] Analyzing Scan Number 1219
[3:42:54 PM] Analyzing Scan Number 1221
[3:42:54 PM] Analyzing Scan Number 1223
[3:42:54 PM] Analyzing Scan Number 1225
[3:42:54 PM] Analyzing Scan Number 1227
[3:42:54 PM] Analyzing Scan Number 1229
[3:42:54 PM] Analyzing Scan Number 1231
[3:42:54 PM] Analyzing Scan Number 1233
[3:42:54 PM] Extracting MS2/3 Data
[3:42:54 PM] Extracted MS2/3 Data
[3:42:54 PM] Analyzing Scan Number 1236
[3:42:54 PM] Analyzing Scan Number 1238
[3:42:54 PM] Analyzing Scan Number 1240
[3:42:54 PM] Analyzing Scan Number 1242
[3:42:54 PM] Analyzing Scan Number 1244
[3:42:54 PM] Analyzing Scan Number 1246
[3:42:54 PM] Analyzing Scan Number 1248
[3:42:54 PM] Analyzing Scan Number 1250
[3:42:54 PM] Analyzing Scan Number 1251
[3:42:54 PM] Analyzing Scan Number 1253
[3:42:54 PM] Analyzing Scan Number 1254
[3:42:54 PM] Analyzing Scan Number 1256
[3:42:54 PM] Analyzing Scan Number 1258
[3:42:54 PM] Analyzing Scan Number 1260
[3:42:54 PM] Analyzing Scan Number 1261
[3:42:54 PM] Analyzing Scan Number 1262
[3:42:54 PM] Analyzing Scan Number 1264
[3:42:54 PM] Extracting MS2/3 Data
[3:42:54 PM] Extracted MS2/3 Data
[3:42:54 PM] Analyzing Scan Number 1265
[3:42:54 PM] Analyzing Scan Number 1268
[3:42:54 PM] Extracting MS2/3 Data
[3:42:54 PM] Extracted MS2/3 Data
[3:42:54 PM] Analyzing Scan Number 1269
[3:42:54 PM] Analyzing Scan Number 1271
[3:42:54 PM] Analyzing Scan Number 1273
[3:42:54 PM] Extracting MS2/3 Data
[3:42:54 PM] Extracted MS2/3 Data
[3:42:54 PM] Analyzing Scan Number 1274
[3:42:54 PM] Analyzing Scan Number 1277
[3:42:54 PM] Extracting MS2/3 Data
[3:42:54 PM] Extracted MS2/3 Data
[3:42:54 PM] Analyzing Scan Number 1278
[3:42:54 PM] Analyzing Scan Number 1281
[3:42:54 PM] Extracting MS2/3 Data
[3:42:54 PM] Extracted MS2/3 Data
[3:42:54 PM] Analyzing Scan Number 1282
[3:42:54 PM] Analyzing Scan Number 1285
[3:42:54 PM] Extracting MS2/3 Data
[3:42:54 PM] Extracted MS2/3 Data
[3:42:54 PM] Analyzing Scan Number 1286
[3:42:54 PM] Analyzing Scan Number 1289
[3:42:54 PM] Extracting MS2/3 Data
[3:42:54 PM] Extracted MS2/3 Data
[3:42:54 PM] Analyzing Scan Number 1290
[3:42:54 PM] Analyzing Scan Number 1293
[3:42:54 PM] Extracting MS2/3 Data
[3:42:54 PM] Extracted MS2/3 Data
[3:42:54 PM] Analyzing Scan Number 1294
[3:42:54 PM] Analyzing Scan Number 1296
[3:42:54 PM] Analyzing Scan Number 1298
[3:42:54 PM] Extracting MS2/3 Data
[3:42:54 PM] Extracted MS2/3 Data
[3:42:54 PM] Analyzing Scan Number 1301
[3:42:54 PM] Analyzing Scan Number 1302
[3:42:54 PM] Analyzing Scan Number 1304
[3:42:54 PM] Analyzing Scan Number 1306
[3:42:54 PM] Analyzing Scan Number 1308
[3:42:54 PM] Analyzing Scan Number 1311
[3:42:54 PM] Analyzing Scan Number 1313
[3:42:54 PM] Analyzing Scan Number 1315
[3:42:54 PM] Analyzing Scan Number 1317
[3:42:54 PM] Analyzing Scan Number 1323
[3:42:54 PM] Analyzing Scan Number 1335
[3:42:54 PM] Analyzing Scan Number 1342
[3:42:54 PM] Analyzing Scan Number 1345
[3:42:54 PM] Analyzing Scan Number 1348
[3:42:54 PM] Analyzing Scan Number 1396
[3:42:54 PM] Analyzing Scan Number 1398
[3:42:54 PM] Analyzing Scan Number 1400
[3:42:54 PM] Analyzing Scan Number 1405
[3:42:54 PM] Analyzing Scan Number 1407
[3:42:54 PM] Analyzing Scan Number 1409
[3:42:54 PM] Analyzing Scan Number 1411
[3:42:54 PM] Analyzing Scan Number 1415
[3:42:54 PM] Analyzing Scan Number 1417
[3:42:54 PM] Analyzing Scan Number 1419
[3:42:54 PM] Analyzing Scan Number 1421
[3:42:54 PM] Analyzing Scan Number 1423
[3:42:54 PM] Analyzing Scan Number 1428
[3:42:54 PM] Analyzing Scan Number 1430
[3:42:54 PM] Analyzing Scan Number 1438
[3:42:54 PM] Analyzing Scan Number 1472
[3:42:54 PM] Analyzing Scan Number 1477
[3:42:54 PM] Analyzing Scan Number 1480
[3:42:54 PM] Extracting MS2/3 Data
[3:42:54 PM] Extracted MS2/3 Data
[3:42:54 PM] Analyzing Scan Number 1483
[3:42:54 PM] Extracting MS2/3 Data
[3:42:54 PM] Extracted MS2/3 Data
[3:42:54 PM] Analyzing Scan Number 1486
[3:42:54 PM] Extracting MS2/3 Data
[3:42:54 PM] Extracted MS2/3 Data
[3:42:54 PM] Analyzing Scan Number 1489
[3:42:54 PM] Extracting MS2/3 Data
[3:42:54 PM] Extracted MS2/3 Data
[3:42:54 PM] Analyzing Scan Number 1490
[3:42:54 PM] Analyzing Scan Number 1493
[3:42:54 PM] Extracting MS2/3 Data
[3:42:54 PM] Extracted MS2/3 Data
[3:42:54 PM] Analyzing Scan Number 1496
[3:42:54 PM] Extracting MS2/3 Data
[3:42:54 PM] Extracted MS2/3 Data
[3:42:54 PM] Analyzing Scan Number 1497
[3:42:54 PM] Analyzing Scan Number 1500
[3:42:54 PM] Extracting MS2/3 Data
[3:42:54 PM] Extracted MS2/3 Data
[3:42:54 PM] Analyzing Scan Number 1503
[3:42:54 PM] Extracting MS2/3 Data
[3:42:54 PM] Extracted MS2/3 Data
[3:42:54 PM] Analyzing Scan Number 1506
[3:42:54 PM] Extracting MS2/3 Data
[3:42:54 PM] Extracted MS2/3 Data
[3:42:54 PM] Analyzing Scan Number 1509
[3:42:54 PM] Extracting MS2/3 Data
[3:42:54 PM] Extracted MS2/3 Data
[3:42:54 PM] Analyzing Scan Number 1512
[3:42:54 PM] Extracting MS2/3 Data
[3:42:54 PM] Extracted MS2/3 Data
[3:42:54 PM] Analyzing Scan Number 1513
[3:42:54 PM] Analyzing Scan Number 1516
[3:42:54 PM] Extracting MS2/3 Data
[3:42:54 PM] Extracted MS2/3 Data
[3:42:54 PM] Analyzing Scan Number 1519
[3:42:54 PM] Extracting MS2/3 Data
[3:42:54 PM] Extracted MS2/3 Data
[3:42:54 PM] Analyzing Scan Number 1522
[3:42:54 PM] Extracting MS2/3 Data
[3:42:54 PM] Extracted MS2/3 Data
[3:42:54 PM] Analyzing Scan Number 1525
[3:42:54 PM] Extracting MS2/3 Data
[3:42:54 PM] Extracted MS2/3 Data
[3:42:54 PM] Analyzing Scan Number 1528
[3:42:54 PM] Extracting MS2/3 Data
[3:42:54 PM] Extracted MS2/3 Data
[3:42:54 PM] Analyzing Scan Number 1529
[3:42:54 PM] Analyzing Scan Number 1532
[3:42:54 PM] Extracting MS2/3 Data
[3:42:54 PM] Extracted MS2/3 Data
[3:42:54 PM] Analyzing Scan Number 1535
[3:42:54 PM] Extracting MS2/3 Data
[3:42:54 PM] Extracted MS2/3 Data
[3:42:54 PM] Analyzing Scan Number 1538
[3:42:54 PM] Extracting MS2/3 Data
[3:42:54 PM] Extracted MS2/3 Data
[3:42:54 PM] Analyzing Scan Number 1541
[3:42:54 PM] Extracting MS2/3 Data
[3:42:54 PM] Extracted MS2/3 Data
[3:42:54 PM] Analyzing Scan Number 1542
[3:42:54 PM] Analyzing Scan Number 1545
[3:42:54 PM] Extracting MS2/3 Data
[3:42:54 PM] Extracted MS2/3 Data
[3:42:54 PM] Analyzing Scan Number 1548
[3:42:54 PM] Extracting MS2/3 Data
[3:42:54 PM] Extracted MS2/3 Data
[3:42:54 PM] Analyzing Scan Number 1549
[3:42:54 PM] Analyzing Scan Number 1552
[3:42:54 PM] Extracting MS2/3 Data
[3:42:54 PM] Extracted MS2/3 Data
[3:42:54 PM] Analyzing Scan Number 1555
[3:42:54 PM] Extracting MS2/3 Data
[3:42:54 PM] No Quant Dict
[3:42:54 PM] MS1 Scan Number1551
[3:42:54 PM] Target Scan Number1557
[3:42:54 PM] MS2 RT10.8632167133333
[3:42:54 PM] MS2 IT900
[3:42:54 PM] MS3 Scan Number1558
[3:42:54 PM] MS3 RT10.8823726850667
[3:42:54 PM] MS3 IT2000
[3:42:54 PM] Adding Target Data 1
[3:42:54 PM] Error! Object reference not set to an instance of an object.
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Hi Chris,
Separate issue.
So I get this error when I try to run Analyze on my desktop PC. All others attempts were done on the acquisition (Fusion Not Lumos) computer. Is there another executable I need on my desktop like MSFileReader? I think I have that but is there something else? Not Registered?
Robert O’Meally, M.S., Proteomics Specialist
Mass Spectrometry and Proteomics Facility
Johns Hopkins School of Medicine
725 N. Wolfe Street, Room 510 Hunterian Bldg.
Baltimore, MD 21205
Ph: (410) 614-6968/Fax: (410) 614-7566
email: romeally@jhmi.edu<mailto:romeally@jhmi.edu>
From: CMRose3355 [mailto:notifications@github.com]
Sent: Monday, July 09, 2018 3:19 PM
To: CMRose3355/TomahaqCompanionProgram <TomahaqCompanionProgram@noreply.github.com>
Cc: Bob O'Meally <romeally@jhmi.edu>; Author <author@noreply.github.com>
Subject: Re: [CMRose3355/TomahaqCompanionProgram] Error Analyzing Data File (#2)
Hello Bob,
Please try the newest version again. The last trial helped be narrow in on the issue. There is a small chunk of code that could be the issue and hopefully the newest version will demonstrate where the error is.
—
You are receiving this because you authored the thread.
Reply to this email directly, view it on GitHub<#2 (comment)>, or mute the thread<https://github.com/notifications/unsubscribe-auth/Am0FZfmZDbEhqYKeZkTyg6FVqUowGePuks5uE6yzgaJpZM4U9VB6>.
[5:06:35 PM] Importing Modifications
[5:06:35 PM] Determining Quantification Channels
[5:06:35 PM] Importing Target Peptides
[5:06:35 PM] Opening Raw File
[5:06:35 PM] Error! Retrieving the COM class factory for component with CLSID {1D23188D-53FE-4C25-B032-DC70ACDBDC02} failed due to the following error: 80040154 Class not registered (Exception from HRESULT: 0x80040154 (REGDB_E_CLASSNOTREG)).
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Yes, you will need MSFileReader including both 32 and 64 bit versions. I will need to send it via email as I don't know if I can host that here on GitHub. |
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Hi Chris,
Did you get the slicer .Raw file I sent you? I am noticing that the .Raw file appears to be only picking one ion for SPS. In the MS3 HCD scans I only see one ion in each scan header. Should there be at least 4 the way I set my method up? I assume you are busy so take your time responding. Just trying to track down where this issue comes from.
Thanks,
Bob
Robert O’Meally, M.S., Proteomics Specialist
Mass Spectrometry and Proteomics Facility
Johns Hopkins School of Medicine
725 N. Wolfe Street, Room 510 Hunterian Bldg.
Baltimore, MD 21205
Ph: (410) 614-6968/Fax: (410) 614-7566
email: romeally@jhmi.edu<mailto:romeally@jhmi.edu>
From: CMRose3355 [mailto:notifications@github.com]
Sent: Monday, July 09, 2018 7:07 PM
To: CMRose3355/TomahaqCompanionProgram <TomahaqCompanionProgram@noreply.github.com>
Cc: Bob O'Meally <romeally@jhmi.edu>; Author <author@noreply.github.com>
Subject: Re: [CMRose3355/TomahaqCompanionProgram] Error Analyzing Data File (#2)
Yes, you will need MSFileReader including both 32 and 64 bit versions. I will need to send it via email as I don't know if I can host that here on GitHub.
—
You are receiving this because you authored the thread.
Reply to this email directly, view it on GitHub<#2 (comment)>, or mute the thread<https://github.com/notifications/unsubscribe-auth/Am0FZSwznkDhH5fLX08967K6ODg_etLNks5uE-H-gaJpZM4U9VB6>.
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I have constructed a method and it runs fine and I can verify the MS3 data by hand, but when I go to analyze the data file in Companion I get an error saying “Error! Object reference not set to an instance of an object.” I have a complex User Mods table where I have to set the Nterm and K as variable but I believe it is set up alright. Can someone tell me what this error might be referring to? I am not sure if it is a bug or something in my method set up, but as I said the method is working I just can't analyze the data. I tried to paste the UserMods as a file but it didn't seem to work so I just pasted the text below. It is complicated but as I said it does work...
Thanks!
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