Add parameter to skip Normalization step?

[mr-september]

I have pre-normalized the data in Seurat, then converted to sce to run DEsingle (as discussed in Seurat's issues, counts=logcounts), but it seems to be re-normalizing the normalized data.

Can an argument be added to DEsingle command to not do further normalization?

[miaozhun]

I suggest to use DEsingle's normalization step with your data or re-normalize the normalized data, since DEsingle's normalization step could ensure the data work with ZINB distribution.