Error when using as.Seurat function

[bapoorva]

Hi,

I tried to convert a seurat object to a single cell experiment and back using the as.SingleCellExperiment and as.Seurat functions and I get he following error when converting back to Seurat

sce= as.SingleCellExperiment(object)
object=as.Seurat(sce)
Error in .subset2(x, i, exact = exact) : subscript out of bounds

It has worked fine before. I recently updated Seurat to version 4. Does it have anything to do with that ?

Thanks for your help.

[andrewwbutler]

Hi,

Are you able to share the object or a reproducible example? That along with the sessionInfo() would be helpful in debugging.