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cutoff parameter does not limit number of cells chosen #40

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kpruss opened this issue Aug 16, 2022 · 0 comments
Open

cutoff parameter does not limit number of cells chosen #40

kpruss opened this issue Aug 16, 2022 · 0 comments

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@kpruss
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kpruss commented Aug 16, 2022

I have not been able to successfully implement a cut-off threshold while calling Scissor.

For example, if alpha = 0.05 leads to 40% of cells being selected by Scissor and I want to decrease the proportion of Scissor-selected cells

cutoff_threshold = 0.2
alpha_threshold = 0.05

info <- Scissor(bulk_dataset = duod_counts,
sc_dataset = seur_duod,
phenotype = phenotype,
alpha = alpha_threshold,
cutoff = cutoff_threshold,
tag = tag,
family = "gaussian",
Save_file = paste0('sbatch_outputs/grid_search/', date, alpha_threshold, "_", cutoff_threshold, protein, '.RData'))

The number and identity of cells selected is exactly the same as that chosen without the inclusion of the cutoff parameter.

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