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{ | ||
"author": "Opentrons", | ||
"categories": { | ||
"Sample Prep": [ | ||
"Plate Filling" | ||
] | ||
}, | ||
"deck-setup": "", | ||
"description": "This protocol preps a 96 200ul Armadillo plate to be used directly on the Cerillo Stratus. For detailed protocol steps, please see below.\nFor an example csv, please see here.\nNote: keep the x in the top left corner. If you would not like to put a volume in a well (ie skipping a well), then replace any number with an \"x\".", | ||
"internal": "cerillo", | ||
"labware": "\nCerillo Stratus Armadillo Flat Bottom Plate 96well 200uL\nNEST 96 Deepwell Plate 2mL #503001\nNEST 12 Well Reservoir 15 mL #360102\nOpentrons 96 Filter Tip Rack 200 \u00b5L\n", | ||
"markdown": { | ||
"author": "[Opentrons](https://opentrons.com/)\n\n\n", | ||
"categories": "* Sample Prep\n\t* Plate Filling\n\n\n", | ||
"deck-setup": "![deck](https://opentrons-protocol-library-website.s3.amazonaws.com/custom-README-images/cerillo/deck.png)\n\n\n", | ||
"description": "This protocol preps a 96 200ul Armadillo plate to be used directly on the Cerillo Stratus. For detailed protocol steps, please see below.\n\n[For an example csv, please see here.](https://opentrons-protocol-library-website.s3.amazonaws.com/custom-README-images/cerillo+csv.xlsx)\n\nNote: keep the x in the top left corner. If you would not like to put a volume in a well (ie skipping a well), then replace any number with an \"x\".\n\n\n", | ||
"internal": "cerillo\n", | ||
"labware": "* Cerillo Stratus Armadillo Flat Bottom Plate 96well 200uL\n* [NEST 96 Deepwell Plate 2mL #503001](http://www.cell-nest.com/page94?product_id=101&_l=en)\n* [NEST 12 Well Reservoir 15 mL #360102](http://www.cell-nest.com/page94?_l=en&product_id=102)\n* Opentrons 96 Filter Tip Rack 200 \u00b5L\n\n\n", | ||
"notes": "If you have any questions about this protocol, please contact the Protocol Development Team by filling out the [Troubleshooting Survey](https://protocol-troubleshooting.paperform.co/).\n\n\n", | ||
"pipettes": "* [Opentrons P300 Single Channel Electronic Pipette (GEN2)](https://shop.opentrons.com/single-channel-electronic-pipette-p20/)\n* [Opentrons P300 8 Channel Electronic Pipette (GEN2)](https://shop.opentrons.com/8-channel-electronic-pipette/)\n\n\n", | ||
"process": "1. Input your protocol parameters above.\n2. Download your protocol and unzip if needed.\n3. Upload your custom labware to the [OT App](https://opentrons.com/ot-app) by navigating to `More` > `Custom Labware` > `Add Labware`, and selecting your labware files (.json extensions) if needed.\n4. Upload your protocol file (.py extension) to the [OT App](https://opentrons.com/ot-app) in the `Protocol` tab.\n5. Set up your deck according to the deck map.\n6. Calibrate your labware, tiprack and pipette using the OT App. For calibration tips, check out our [support articles](https://support.opentrons.com/en/collections/1559720-guide-for-getting-started-with-the-ot-2).\n7. Hit \"Run\".\n\n\n", | ||
"protocol-steps": "1. If user decides, buffer is added to deepwell plate according to buffer csv (single-channel).\n2. If user decides, stock is added to deepwell plate according to stock csv (single-channel).\n3. Stock and buffer are mixed with multi-channel pipette, then transferred to reader.\n4. Cells premixed with mulit-channel.\n5. 180ul of cells are transferred to reader, mixing 3 times when mixed in diluted stock. \n\n\n\n", | ||
"title": "Cerillo Plate Reader Protocol" | ||
}, | ||
"notes": "If you have any questions about this protocol, please contact the Protocol Development Team by filling out the Troubleshooting Survey.", | ||
"pipettes": "\nOpentrons P300 Single Channel Electronic Pipette (GEN2)\nOpentrons P300 8 Channel Electronic Pipette (GEN2)\n", | ||
"process": "\nInput your protocol parameters above.\nDownload your protocol and unzip if needed.\nUpload your custom labware to the OT App by navigating to More > Custom Labware > Add Labware, and selecting your labware files (.json extensions) if needed.\nUpload your protocol file (.py extension) to the OT App in the Protocol tab.\nSet up your deck according to the deck map.\nCalibrate your labware, tiprack and pipette using the OT App. For calibration tips, check out our support articles.\nHit \"Run\".\n", | ||
"protocol-steps": "\nIf user decides, buffer is added to deepwell plate according to buffer csv (single-channel).\nIf user decides, stock is added to deepwell plate according to stock csv (single-channel).\nStock and buffer are mixed with multi-channel pipette, then transferred to reader.\nCells premixed with mulit-channel.\n180ul of cells are transferred to reader, mixing 3 times when mixed in diluted stock. \n", | ||
"title": "Cerillo Plate Reader Protocol" | ||
} |
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