Running Pipelines

How to run pipelines in the portal.

We currently have the ability to run pipelines in the portal!!!! These pipelines run in a cloud environment (Google Cloud Platform) and are intended to give an easy experience from running pipelines using raw files (currently fastq.gz) to offering a default interactive view of the study quickly. We are excited to make life easier!

Eventually, you will need a billing object to run pipelines but we offer free credits to get you going :-)

Step 0. Contact us to get access as an alpha user.

Email us at single_cell_portal@broadinstitute.org

Step 1. Get free credits!

Please go here to apply for free credits..

If set up correctly, free credits in FireCloud will work as free credits in the Singel Cell Portal.

Step 2. Create a study

You will first need to create a study so there is a place to work with your file.

Please see instructions here.

Step 3. Upload data to a study

You will need to upload your raw data to your bucket associated with the study.

Here are instructions to upload to your bucket using gsutil.

Please note, it may be easier for you long term to collect all your fastqs.gz files in a "folder" in the bucket. This is simply done by adding the directory you want to copy to in the gsutil cp command (the directory will automatically be made for you).

An example of this if you were wanting to copy all fastq.gz files in your current directory and put them in the "fastqs" directory in the bucket.